sc 271300 (Santa Cruz Biotechnology)
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Structured Review
Santa Cruz Biotechnology
sc 271300
Sc 271300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+271301/Munc13-4+Antibody/pm41083534-565-13-11
Average 93 stars, based on 14 article reviews
Sc 271300, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+271301/Munc13-4+Antibody/pm41083534-565-13-11
Average 93 stars, based on 14 article reviews
sc 271300 - by Bioz Stars,
2026-09
93/100 stars
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Incubation:Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes. Article Snippet: Equal numbers of SUM159 cells were seeded onto 15-cm culture dishes, with one plate treated with 100 ng/ ml IFNγ (ABclonal, RP01038) for 24 h. Following the incubation, cells were lysed on ice using RIPA buffer (50mM Tris-HCl, pH 7.5, 150mM NaCl, 1% TritonX-100) supplementedwith a protease inhibitor cocktail (Topscience, C0001). .. The lysates were then centrifuged at 4 °C for 10min at 12,000 × g. The supernatant was incubated overnight at 4 °C with Munc13-4 Antibody (C-12) (Santa Cruz, Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes Article Snippet: Following the incubation, cells were lysed on ice using RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100) supplemented with a protease inhibitor cocktail (Topscience, C0001). .. The lysates were then centrifuged at 4°C for 10 minutes at 12,000 × g. The supernatant was incubated overnight at 4°C with Munc13-4 Antibody (C-12) (Santa Cruz, Magnetic Beads:Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes Article Snippet: Following the incubation, cells were lysed on ice using RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100) supplemented with a protease inhibitor cocktail (Topscience, C0001). .. The lysates were then centrifuged at 4°C for 10 minutes at 12,000 × g. The supernatant was incubated overnight at 4°C with Munc13-4 Antibody (C-12) (Santa Cruz, Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes Article Snippet: Equal numbers of SUM159 cells were seeded onto 15-cm culture dishes, with one plate treated with 100 ng/ml IFNγ (ABclonal, RP01038) for 24 h. Following the incubation, cells were lysed on ice using RIPA buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100) supplemented with a protease inhibitor cocktail (Topscience, C0001). .. The lysates were then centrifuged at 4 °C for 10 min at 12,000 × g . The supernatant was incubated overnight at 4 °C with Munc13-4 Antibody (C-12) (Santa Cruz, |